Services

The Genomics Core facility, located in Sanger Hall and in the VCU Health Science Research Building on the MCV campus, maintains a significant portfolio of instrumentation to support advanced sample and library preparation, high-throughput sequencing, real-time PCR and analysis. In addition to a broad range of instruments for sample preparation and quality control instrumentation, key platforms include:

Chromium X (10x Genomics)
Visium CytAssits (10x Genomics)*
NextSeq2000 Sequencer (Illumina)
MiSeq Sequencer (Illumina)
ViiA 7 Real-Time PCR system (ThermoFisher Scientific)
QIAcuity digital PCR (Qiagen)

The Illumina platforms offer a large variety of sequencing configurations ranging from 1 x 50 bp single end (SE) to 2 x 300 bp paired end (PE) or 1 x 600 bp single end (SE) sequencing, generating up to 1.8 billion read pairs per sequencing run.

Typical services:

DNA and/or RNA extraction
de novo sequencing
whole genome sequencing (WGS)
whole exome sequencing (WES)
whole genome bisulfite sequencing (WGBS)
methyl-binding domain sequencing (MBD-seq)
transcriptome sequencing based on poly-A enrichment (RNA-seq)
total RNA sequencing with ribosomal depletion (RNA-seq)
small RNA sequencing (microRNA-seq)
circular RNA sequencing (circRNA-seq)
chromatin immunoprecipitation sequencing (ChIP-seq)
assay for transposase-accessible chromatin sequencing (ATAC-seq)

amplicon sequencing
mitochondrial sequencing
16 S rRNA taxonomic profiling
metagenome sequencing
meta-transcriptome sequencing
single cell/nucleus RNA sequencing (_sc/nRNA-seq)
single cell/nucleus ATAC sequencing (_sc/nATAC-seq)
single cell/nucleus multiomic sequencing (_sc/n-seq)
spatial transcriptomics
C. bovis detection with real-time qPCR
Custom qPCR assays

The VCU Genomics Core works closely with other VCU facilities, to allow for
streamlined support for tissue sectioning and staining (Tissue and Data
Acquisition and Analysis), data analysis (Bioinformatics Shared Resource)
and data storage (VCU High Performance Research Computing Core).