Services
The Genomics Core facility, located in Sanger Hall on the MCV campus, maintains a significant portfolio of instrumentation to support high-throughput sequencing and analysis. In addition to a broad range of instruments for sample preparation, library construction and quality control, the core hosts a number of sequencing platforms:
- Illumina NextSeq2000 – NEW in Spring 2021
- Illumina MiSeq
- Illumina HiSeq4000 (to be phased out in Summer 2021)
- Pacific Biosciences Sequel
The Illumina platforms offer a large variety of sequencing configurations ranging from 1 x 50 bp single end (SE) to 2 x 300 bp paired end (PE) sequencing and the Sequel instrument provides long reads of up to 100 kilobases.
Typical services:
- de novo sequencing
- whole genome sequencing (WGS)
- whole exome sequencing (WES)
- whole genome bisulfite sequencing (WGBS)
- methyl-binding domain sequencing (MBD-seq)
- transcriptome sequencing (RNA-seq)
- small RNA-seq (microRNA, non-coding RNAs)
- chromatin immunoprecipitation sequencing (ChIP-seq)
- targeted genome sequencing
- amplicon sequencing
- mitochondrial sequencing
- 16 S rRNA taxonomic profiling
- metagenome sequencing
- metatranscriptome sequencing
- long read sequencing
In collaboration with other VCU facilities, the core offers state-of-the-art single cell sequencing using a Chromium system from 10X Genomics; anticipates the ability to provide spatial transcriptomic analysis in Summer 2021; and provides multi-petabyte level data storage for genomic data with access to dedicated server clusters for genomic analyses in the VCU High Performance Research Computing Core.
Location
1101 E Marshall St
Sanger Hall
Rm. 5-028
Richmond, VA 23298
genomics@vcu.edu
Contact us
Karolina A. Aberg, Ph.D.
Co-director
(804) 628-3023
Gregory A. Buck, Ph.D.
Co-director
(804) 828-2318
