Services
The Genomics Core facility, located in Sanger Hall and in the VCU Health Science Research Building on the MCV campus, maintains a significant portfolio of instrumentation to support advanced sample and library preparation, high-throughput sequencing, real-time PCR and analysis. In addition to a broad range of instruments for sample preparation and quality control instrumentation, key platforms include:
- Chromium X (10 Genomics)
- NextSeq2000 Sequencer (Illumina)
- MiSeq Sequencer (Illumina)
- ViiA 7 Real-Time PCR system (ThermoFisher Scientific)
The Illumina platforms offer a large variety of sequencing configurations ranging from 1 x 50 bp single end (SE) to 2 x 300 bp paired end (PE) or 1 x 600 bp single end (SE) sequencing, generating up to 1.2 billion read pairs per sequencing run.
Typical services:
- DNA and/or RNA extraction
- de novo sequencing
- whole genome sequencing (WGS)
- whole exome sequencing (WES)
- whole genome bisulfite sequencing (WGBS)
- methyl-binding domain sequencing (MBD-seq)
- transcriptome sequencing based on poly-A enrichment (RNA-seq)
- total RNA sequencing with ribosomal depletion (RNA-seq)
- small RNA sequencing (microRNA-seq)
- circular RNA sequencing (circRNA-seq)
- chromatin immunoprecipitation sequencing (ChIP-seq)
- assay for transposase-accessible chromatin sequencing (ATAC-seq)
- amplicon sequencing
- mitochondrial sequencing
- 16 S rRNA taxonomic profiling
- metagenome sequencing
- metatranscriptome sequencing
- single cell/nucleus RNA sequencing (sc/nRNA-seq)
- single cell/nucleus ATAC sequencing (sc/nATAC-seq)
- single cell/nucleus multiomic sequencing (sc/n-seq)
- C. bovis detection with real-time qPCR
- Custom qPCR assays
In collaboration with other VCU facilities, the core offers support for library preparation and sequencing of various spatial transcriptomic analysis approaches such as 10x Genomics Visium and NanoString GeoMx; and provides multi-petabyte level data storage for genomic data with access to dedicated server clusters for genomic analyses in the VCU High Performance Research Computing Core.