Bioimaging and Applied Research Core
The Bioimaging and Applied Research Core (BARC) provides state-of-the-art imaging technologies to foster multi-modality approaches to study anatomy, biology, biochemistry, and pharmacology in vivo and in vitro.
About
The Bioimaging and Applied Research Core, directed by Frank Corwin, Ph.D. and managed by Mackenzie Newman, Ph.D., offers comprehensive instrumentation and technical support for all aspects of in vitro and in vivo pre-clinical non-human anatomical and molecular imaging. Studies may be conducted on samples prepared to exhibit characteristic features for a particular imaging modality. Within the intact environment, physiological and pathophysiological activity may be observed through imaging or numerous biological pathways and interactions involved in injury and disease progression, such as traumatic brain injury, cardiac disease, tumorigenesis, and therapeutic interventions.
BARC offers a wide variety of services via a traditional fee-for-service model and collaborative solutions that involve do-it-yourself imaging and data analysis and lab-expertise for hire. These models give investigators the opportunity for training and hands-on time on selected instrumentation and permit investigators to purchase technician time from experienced staff to contribute to protocol development and optimization of new approaches. Staff can assist in experimental planning and project design.
News
12/6/24: Paravision 7 and the proton density fat fraction package have been installed on our Bruker Biospec 7T. These upgrades will help streamline future MR data acquisition and expand our capacity for quantitative tissue analysis. Please inquire for more detail!
10/31/24: Our services request form is now live! We are happy to continue communicating via email, but this will help streamline the intake process, especially for larger studies or those that may require add-on services beyond imaging.
6/5/24: BARC has been approved to purchase a new laser for our iThera Medical MSOT inVision system! This will extend our maximum excitation wavelength from 1000 to 1300nm, well into infrared-A, allowing imaging of collagen deposition typically associated with fibrosis.
Initiate a project
BARC offers a new business model based on a broad range of off-the-shelf, state-of-the-art services as well as the ability to develop and tailor protocols for specific research projects. To initiate a service request or to schedule a discussion about an ongoing or upcoming project please use our intake form or contact us via email.
Suggested publication language
Published work using data generated at BARC should include the following in the acknowledgment:
The data included in this study was generated at the Bioimaging and Applied Research Core facility at Virginia Commonwealth University.
Suggested grant language
Include the following text in the Facilities and Resources section of grant applications when proposing to conduct work that utilizes the BARC facility:
The Bioimaging and Applied Research Core laboratory (BARC) is a VCU institutionally-designated shared resource providing state-of-the-art imaging technologies to support research in life and physical sciences, with an emphasis on multi-modality imaging approaches to study structure, biology, biochemistry and pharmacology in vitro and in vivo.
The BARC is directed by Frank Corwin, Ph.D., and managed by Mackenzie Newman, Ph.D., who have more than 30 years of combined experience in the field of non-invasive in vivo imaging. The BARC provides a hub for translational research, with molecular imaging equipment that supports imaging of small mammals, as well as supporting physical sciences applications such as fluid dynamics.
Our shared resource facility for anatomical and molecular imaging increases the ability of investigators to translate preclinical concepts of injury and/or cancer into detection, therapy, and remission in animal model systems and, ultimately, into early phase clinical trials. Further, it provides expertise and instruments that facilitate direct, specific, and ultra-sensitive studies of molecular pathways in the in vivo environment. This always-expanding core leverages multiple imaging technologies, combined with vendor-supplied imaging probes and contrast agents, available through VCU BARC.
The BARC offers radionuclide imaging services of small animals on PET as well as non-radionuclide-based imaging using MRI/MRS, photoacoustic, and multispectral fluorescence systems. The facility also provides project consultation, user training, and staff-assisted data analysis services.
Located on the VCU MCV campus, the BARC maintains a significant portfolio of instrumentation in support of in vitro and in vivo imaging. The state-of-the-art imaging equipment includes:
- Bruker Biospec 7 Tesla preclinical magnetic resonance imager system, with multinuclear capability
- LFER PET/CT in-vivo animal imager (Mediso USA), capable of large field-of-view (15 cm axial, 20 cm trans-axial), whole body preclinical imaging
- Multispectral optoacoustic tomography (MSOT) system (iThera Medical GmBH, Munich, Germany)
- Maestro-2 multispectral small animal imaging system (CRi Inc).
Commonly provided services include:
- Training on the operation of the imaging modalities
- Direct operations and data analysis support on the more complex modalities
- Project design and implementation guidance
Additionally, BARC has immediate access to separate animal holding facilities available adjacent to the imaging suites, which are approved to house animals injected with radioactive substances. Sufficient space for housing mice and rats is available and includes cages with filter tops, laminar flow racks, and biosafety cabinets to prevent the spread of infectious disease. The VCU Division of Animal Resources provides animal care 365 days a year. Veterinary consultation services are readily available, as well as training and technical services regarding surgery and blood sampling.
Recent citations
Wiggins, C.S., Cabral, A., Mafi, A. et al. Integrated positron emission particle tracking (PEPT) and X-ray computed tomography (CT) imaging of flow phenomena in twisted tape swirl flow. Exp Fluids 65, 121 (2024). https://doi.org/10.1007/s00348-024-03860-7
India K Hawkins, Josh Mitchell, Adolfo G Mauro, Nigeste M Carter, Frank Corwin, Fadi N Salloum, Frank J Raucci. "Measuring Fibrosis Progression in Duchenne Cardiomyopathy Using Cardiac Magnetic Resonance in mice". Basic Cardiovascular Sciences Conference, 22-25 July 2024.
Irwin, L. A., Lee, L., Mitchell, J., Corwin, F. D., Coelho, D. H., & Manzoor, N. F. (2024). Endoscopic-Assisted Presigmoid Approach to the Internal Auditory Canal. A Feasibility Study. In Otology & Neurotology (Vol. 45, Issue 7, pp. 806–809). Ovid Technologies (Wolters Kluwer Health). https://doi.org/10.1097/mao.0000000000004248
Wiggins, C. S., Cabral, A., & Carasik, L. B. (2023). Coregistered positron emission particle tracking (PEPT) and X-ray computed tomography (CT) for engineering flow measurements. In Nuclear Engineering and Design (Vol. 403, p. 112125). Elsevier BV. https://doi.org/10.1016/j.nucengdes.2022.112125
Brøndsted, F., Fang, Y., Li, L., Zhou, X., Grant, S., & Stains, C. I. (2023). Single Atom Stabilization of Phosphinate Ester‐Containing Rhodamines Yields Cell Permeable Probes for Turn‐On Photoacoustic Imaging**. In Chemistry – A European Journal (Vol. 30, Issue 1). Wiley. https://doi.org/10.1002/chem.202303038